PRISM Visits the NYC Office of Chief Medical Examiner (OCME)


On January 26th, 10 PRISM students had the opportunity of visiting the Forensic Biology Department of the Office of Chief Medical Examiner.  Scientists there demonstrated how samples like DNA are extracted and analyzed directly from evidence.  Students also had the opportunity to hear about careers at the OCME. 

From the OCME website: The OCME investigates cases of persons who die within New York City from criminal violence, by accident, by suicide, suddenly when in apparent health, when unattended by a physician, in a correctional facility or in any suspicious or unusual manner or where an application is made pursuant to law for a permit to cremate a body of a person.  In addition, the OCME Forensic Biology lab also tests thousands of items of evidence each year from nearly every category of crime including homicide, sexual assault, felony assault, robbery, burglary, hate crimes, and weapons possession. The lab also performs testing associated with missing persons, unidentified remains, and the ongoing effort to identify World Trade Center victims.

PRISM Students Shine at the Annual Biomedical Research Conference for Minority Students (ABRCMS) 2015


16 PRISM students attended the ABRcMS conference this past November in Seattle Washington to present in oral and poster presentations of their research projects. Nine of these students received travel awards to attend.  Three of our students, Ronald Rodriguez, William Aguilar and Jiwon Seo, won for their presentations.  The conference lasted over 4 days and over 1,700 undergraduate and graduate students participated in this annual conference.   The goal of the conference is to increase underrepresented minority students in the STEM (Science, Technology, Engineering and Math) fields, specifically encouraging them to pursue advanced degrees. In addition, workshops are provided for faculty and program coordinators to provide them with resources that promote students success.

Students were accompanied by the PRISM Program and Research Coordinator, Dr. Edgardo Sanabria-Valentin, Dr. Nathan Lents and Dr. Jason Rauceo.

For more pictures visit:

The attendees included:

  • William Aguilar (Dr. Elise Champeil) – Correlation of Mc and Dmc-Adducts Structures with the Role of P21 in the Toxicity of the α-icl and β-ic
    *Winner of a Chemistry Oral Presentation Award
  • Ronald Rodriguez (Dr. Jason Rauceo) – Localization Analysis of a Major Osmotic Stress Response Gene in the Fungus Candida albicans
    *Winner of a Microbiology Oral Presentation Award
  • Jiwon Seo (Dr. Shu-Yuan Cheng) – The Effects of Upstream Nucleotides on the Readthrough Rate of VEGF-Ax
    *Winner of a Biochemistry Poster Presentation Award
  • Daysi Proano (Dr. Jason Rauceo) – Characterizing MHCI cytoplasmic domain phosphorylation in vitro and its role in binding interactions
  • Shanelle Shillingford (Dr. Gloria Proni) – The Oxidation of Allylic Alcohols to Ketones with the use of Electrochemistry
  • David Rodriguez (Dr.  Artem Domashevskiy) – Synthesis of Fluorescently-Labeled Tobacco Etch Virus (TEV) RNA and its Interactions with Pokeweed Antiviral Protein (PAP)
  • Donovan Trinidad (Dr. Nathan Lents) – Analysis of the Human Microbiome on Living and Decomposing Bodies
  • Imani Hargett (Dr. Lissette Delgado-Cruzata) – Understanding the Role of TET Proteins in 5-Hydroxymethylation in Hepatocellular Carcinoma
  • Erica Klafehn (Dr. Angelique Corthals) – 2D to 3D Rending of Bones Samples from Sus scrofa
  • Danielle Rouse (Dr. Anthony Carpi) – The Reduction Mechanism of Mercuric Oxide in the Environment
  • Sabrina De Los Santos (Dr. Artem Domashevskiy) – Analysis of the Effects of Turnip Mosaic Virus Protein-Linked Genome on Ricin a Chain Depurination of Eukaryotic Ribosomal RNA
  • Yuanzhuo “Jove” Zhang (Dr. Guoqi Zhang) –  Diverse Copper(ii) Complexes with Simple Nitrogen Ligands: Structural Characterization and Applications in Aerobic Alcohol Oxidations in Water
  • Ronal Peralta (Dr. Anthony Carpi) – Using Moss as a Biomonitor to Detect Atmospheric Sources of Mercury
  • Lisset Duran (Dr. Lissette Delgado-Cruzata) – Studying the Loss of DNA Methyltransferase DNMT1 in BRCA1 Expression in Breast Cancer
  • Porfirio Fernandez (Dr. Jason Rauceo) – Behavior Expression Profile of alpha-Conotoxin PeI A in Chronic Constriction Injury Model in Rats
  • Maria Anaya (Dr. Areti Tsimounis-QCC)- Morphological characterization of supragranular neurons in the primary somatosensory cortex


PRISM Represents John Jay College at the State Capitol

PRISM Represents John Jay College at the State Capitol

In honor of John Jay’s 50th anniversary, the New York State Assembly and Senate passed resolutions to recognize the College for all of its accomplishments, progress, contributions to student success and justice advocacy.

PRISM had the great honor of representing John Jay during these proceedings.  We are very grateful to our hosts and sponsors, Assemblywoman Linda Rosenthal and Senator Brad Hoylman.

(06-09-15) 27-06-0006

20 PRISM students had their research posters displayed near the legislative offices and enjoyed speaking with elected officials, lobbyists, and capitol visitors about their work.  PRISM was also represented by three of its faculty mentors – Professors Artem Domashevskiy, Angelique Corthals, and Shu-Yuan Cheng.  When not chatting about their research, the PRISM contingent sat in to hear the resolutions and received personal recognition in both chambers of the State Congress.  Many students and accompanying faculty mentors were able to meet their representatives and speak in depth about their research and academic goals.

The students and faculty mentors in attendance made PRISM proud for the way in which they represented John IMG_3820Jay and should also be commended for their resilience during the long trip back and forth to Albany.  The trip was a memorable one for many reasons, including three bus breakdowns!

Click here for more pictures!

PRISM Students and Mentor, Dr. Yi He, Conduct Research at USDA Agricultural Research Service (ARS)

PRISM Students and Mentor, Dr. Yi He, Conduct Research at USDA Agricultural Research Service (ARS)

Early this summer, PRISM mentor, Dr. Yi He, recipient of the prestigious, 2015 USDA, E. Kika De La Garza Fellowship Program, afforded five of her lab students, one week of technical training at the USDAimage6 (2)  Agricultural Research Service (ARS) laboratory, located in Beltsville, MD. ARS is one of the world’s premier scientific  organizations. The E. Kika De La Garza Fellowship Program offers faculty and staff from HSIs the opportunity to work collaboratively with USDA to address the challenges faced in the development of a well prepared Hispanic workforce. Dr. He and her students were provided the opportunity to further investigate toxic heavy metals in tobacco products.  PRISM attendees included (l-r in picture at right) Colleen McNamara, Kathleen Lopez, Dr. Yi He, Victoria Mei, Fidelis Tan, and Tiffany Wong.

Students were able to learn lab protocols for conducting trace metal analysis and for using a dry ashing method, and a microwave digestion technique to process plant samples for inductively coupled plasma-optical emission spectroscopy (ICP-OES) analysis. All students had hands-on experience in the lab and worked side-by-side with leading research scientists who conduct research in toxic heavy metals. The students training, experience and exposure afforded by Dr. He’s fellowship is immeasurable.

Dr. Rufus Chaney, USDA, Research Agronomist, Crop Systems & Global Change was their host scientist and gave the students a presentation about lead (Pb) in garden soil, discussed metal distribution in some vegetables, how to prevent Pb exposure to young children, and exchanged ideas on how to prepare soil to start a new garden. 20150708_125153 (2)In addition to learning new technical lab protocols, the students were impressed by how the lab was organized, and discussed how they can improve organizing their lab drawers back at JJAY. Students also visited a plant growth chamber and greenhouse where Dr. Carrie Green introduced current experiments and other interesting projects conducted by other researchers in ARS.  Students showed special interest in  heavy metal hypercumulating plants and the concept of “Mine Plant,” which means the metal concentration in plant is so high that the plant can be used as the source of mine. Students are anxious to get back to work at JJAY, to apply all that they learned.

Below read some of their accounts of the experience:

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Fidelis Tan

“Working for the USDA was a fun and new experience for me since I got to visit one of the government laboratories and I also had the opportunity to work closely with Dr. Green, the lab manager of the environmental laboratory. At the laboratory, I did a lot of acid cleaning and preparations prior to the analysis. Even though these tasks may seem petty, it is the most critical part for trace metal analysis/research since the quality of data relies on minimal sources of error, and proper sterilization protocol plays a huge part in such. Along with that, I learned to use the microwave digestion system, which involves using organic molecules to dissolve our tobacco samples prior to analysis using ICP-OES (Inductively coupled plasma-optical emission spectrometry). Interning in the USDA laboratory helped expand my research skills at John Jay since I got to learn about the significance of each step involved in the experiments and how will it affect the final results –  especially when handling over 100 samples.”  – Fidelis Continue reading

PRISM Sponsored Summer GRE Course

Mark Your Calendars for PRISM-Sponsored GRE Prep Course

All junior and senior level students with plans to attend graduate school are expected to sign up for and attend Dr. Pilette’s summer GRE Prep Course.  It will run mid-June through July on Fridays at John Jay.  This is an amazing opportunity for you to receive expert test prep guidance at no cost to you.  This is a full course with the preparation book also provided to you at no cost.  You can sign up by emailing Dr. Pilette at  Anyone interested but with a schedule conflict should contact Dr. Pilette to discuss potential alternative arrangements.  Sign up ASAP before seats fill up.

PRISM Attends ASBMB Experimental Biology Conference in Boston, MA

PRISM Attends the ASBMB Experimental Biology Conference in Boston, MA

with signThe ASBMB Experimental Biology (EB) conference is one of the biggest annual biology-based conferences in the country and this year it took place from March 28-April 1 in Boston, MA.  The presentation categories were varied, including such topics as DNA replication studies and cancer genetics. Along with presenters and students, vendors from all around the country set up booths to advertise their cutting-edge instrumentation and kits.  In addition to lectures, vendors, and presentations, this conference offers interactive seminars on such topics as “how to write your resume” and “how to effectively apply for graduate schools.”

This year, PRISM sent two members with mentor, Prof. Artem Domashevskiy, to the EB Conference.  Valentina Aitbakieva presented a poster on her work, “Isolation and Characterization of Different Isoforms of Pokeweed Antiviral Protein (PAP) from Phytolacca americana,”  and David Rodriguez presented a poster on his work, “Studying Interactions Between Pokeweed Antiviral Protein (PAP) and Fluorescently Labeled Tobacco Etch Virus RNA.”  PRISM was also able to send an additional 3 students this year, Karen Conrad, Porfirio Fernandez, and Derek Sokolowski as conference participants even though they did not present their work.

David Rodriguez is a junior student in the PRISM program and this was his first experience presenting at a professional conference.  Below is his account:

Dom and Rod“It was an intimidating experience but an absolute pleasure to attend and present at the 2015 Experimental Biology conference in Boston. The scale of the conference could be felt immediately by the sheer number of people just waiting to get their name badges.  This feeling was amplified further when I entered the grand hall where most of the conference was held. Though a little nervous, my colleagues and I dove right in. We spent the several days talking to other students, top scientists, and vendors from all over the country. One of the most inspiring things I noticed was the variety of research going on. The amount of imagination and intelligence that went into some of the research I learned about is nothing short of amazing. I spent the last day of my visit presenting my own research. I had never presented my research to this point, and I was nervous about my first time being at such a huge conference with so many brilliant people who specialize in biology. Luckily I had been preparing with the help of my mentor, friends, and PRISM. Though nervous. I concentrated and presented my research to quite a few very knowledgeable people. When it was all over, I felt a great sense of accomplishment and was actually excited to present my research and interact with interested people again. I can’t wait until next year.”

PRISM Holds Its 8th Annual Symposium of Undergraduate Research

PRISM Symposium Highlights

Posters CISA recogroup picrd number of PRISM students (46) participated in this year’s 2015 Symposium on Wednesday, April 29. Projects for every interest and for every track were displayed.  This year’s Symposium showcased our growing population of computer science majors as well as two of our CUNY Justice Academy partner students who perform their research with mentors at Queensborough Community College.  Students impressed the guests with their research accomplishments and their ability to engage others when speaking about their work.  We were treated to a visit by Provost Bowers and President Travis, the latter of whom addressed students warmly and hailed their accomplishments.

After the poster session, there were two oral presentations: Yessenia Lopez, Outstanding Undergraduate Researcher and keynote speaker, Dr. Daniel Cocris.

Dr. Demi Cheng was thrilled to introduce her mentee, Yessenia Lopez, to kick off the presentations. Yessenia then showcased her research, “Manganese-Containing Dithiocarbamate Pesticides Increase B-Amyloid Precursor Protein and B-Amyloid Peptide Expression in PC-12 Cells.”  During her presentation, Yessenia gave the full story of her research journey, noting the significant impact of the deep mentoring she received from Dr. Cheng.

CocrisDr. Daniel Cocris then took over and inspired the audience by talking about his entire journey from leaving his home in Romania to finding his stride at John Jay performing research with Dr. Anthony Carpi.  Dr. Cocris spoke about the value of discovering his niche and his love of science, and about the labor involved to achieve his current success as a dentist.


Dr. Anthony Carpi served as the Master of Ceremonies at the PRISM Awards Ceremony, handing out certificatesHooding to all who participated and special awards to our speakers and poster winners.  This year PRISM honored its graduating seniors.  As theses titles were announced, mentors presented these students with PRISM stoles to be worn at graduation.

Awards Presented:

Recognition as Keynote Speaker:  Dr. Daniel Cocris

Outstanding Undergraduate Researcher: Yessenia Lopez

First Place – Poster Contest: Derek Sokolowski

Runners-Up – Poster Contest:  Karen Conrad, Richard Khusial, and Shawn Williams








For more pictures from this event, please click here.

Congratulations to PRISM’s 2015 Outstanding Undergraduate Researcher – Yessenia Lopez

P1000652Yessenia Lopez is this year’s honored recipient of PRISM’s Outstanding Undergraduate Researcher Award.  She joined the lab of Dr. Demi Cheng in June 2013 to pursue a project related to Alzheimer’s Disease, seeking to determine if the pesticides maneb (MB) and mancozeb (MZ) increase the expressions of β-amyloid precursor protein (AβPP) and β-amyloid peptide 42 (Aβ42). During her time in Dr. Cheng’s lab, Yessenia transformed from a science student into young scientist, showcasing her ability to think critically, work independently, and collaborate effectively.  In the words of her mentor, “She takes pride in her lab work, she gets truly excited about results, and the high quality of her research reflects this.” Yessenia has accepted an offer to participate in the competitive post-baccalaureate PREP program at Albert Einstein College of Medicine.  This is her next step on the way to pursuing her PhD and fulfilling her career goal to be an independent scientist.

American Society of Crime Laboratory Directors – Undergraduate Scholarship

The ASCLD’s annual scholarship solicitation is now open and accepting applications.  The annual award is in the amount of $1000 and the  due date (April 15) is quickly approaching.  The application can be found on the ASCLD website. A description of the scholarship can be found here.

Applicants must be a junior or senior in a baccalaureate program or a graduate student (masters or doctorate) at an accredited university who is pursuing a degree in forensic science, forensic chemistry, a physical or natural science.  Applicants from FEPAC accredited programs will be given additional consideration.  High school students or college/university students in their freshman or sophomore years will not be eligible for application.  As the scholarship program was created to provide opportunity to students intending to enter the forensic field, scholarships will not be granted to current forensic science laboratory employees pursing graduate degrees.

Selection criteria
Award recipients will be selected on the basis of:

  • Overall scholastic record
  • Scholastic record in forensic science coursework
  • Motivation or commitment to a forensic science career
  • Personal statement
  • Faculty/adviser recommendation

Application documentation

  • Application form
  • Transcripts
  • Personal statement of applicant
  • Recommendation of faculty member or lab director with knowledge of the applicant

Good Luck!

PRISM students accepted to multiple programs this summer and next year

As of fall 2014, 51 PRISM alumni reported gaining admission to graduate and professional programs since 2006. This year four of our seniors join this group as they enter graduate and medical school programs:


Williams, ShawnShawn Williams (Mentor: Dr. Domashevskiy):

Molecular Biology, Cell Biology & Biochemistry PhD Program, Brown University



image1Tanya Napolitano (Mentors: Drs. Proni & Petraco):

College of Pharmacy and Health Sciences – Toxicology PhD Program, St. John’s University



Reinfeld, SamSamuel Reinfeld (Mentor: Dr. Domashevskiy):

Doctor of Osteopathic Medicine Program (DO), NY College of Osteopathic Medicine



P1000642Yessenia Lopez  (Mentor: Dr. Cheng):

NIH Funded Post Baccalaureate Research Preparation Program (PREP), Albert Einstein College of Medicine


In addition, six PRISM juniors will be attending prestigious summer research programs with various universities and institutes this summer:


Fernandez, PorfirioPorfirio Fernandez (Mentor: Dr. Rauceo):

Leadership Alliance/Howard Hughes Medical Institute Summer Undergraduate Research Program, University of Miami



PROANO_DAYSI croppedDaysi Proano (Mentor: Dr. Svoronos – QCC):

Undergraduate Summer Research in Molecular Biophysics, Princeton University



Seo, JiwonJiwon Seo (Mentor: Dr. Cheng):

Summer Research Opportunities at Harvard (SROH) Program, Harvard University



Shillingford, Shanelle croppedShanelle Shillingford (Mentor: Dr. Proni):

2015 Summer Undergraduate Research Fellows (SURF) Program, The Scripps Research Institute (California campus)



Williams, Desiree croppedDesiree Williams (Mentor: Dr. Li):

Summer Undergraduate Research Program, City University of New York



Duran, Lissette.croppedLisset Duran (Mentor: Dr. Lissette Delgado Cruzata):

Morocco Summer Research Program, National Institute of Medicinal and Aromatic Plants



Good luck on your endeavors! We are very proud that you are all part of our PRISM family!

PRISM Welcomes Its Newest Members!

Fifteen new recruits are now part of our official PRISM family after having attended the Research Training Workshop (RTW) this past January 20-22.

RTW gang jan 2015

Our newest members are from left to right, above: Nithusha Mayakaduwage, Imani Hargett, Laura Duffy, Brianna Bell, Elmer Morales, Jia Wen Tan, Michael Wu, Zenab Khan, William Aguilar, Jeanine Day, Donovan Trinidad, Gerallynn Eusebio, Ronald Peralta, Khamattie Uzagir, and Brooke Nielsen.  Each trainee met and exceeded our minimum PRISM requirements as there was stiff competition this winter for our limited RTW spots.  Among the students were three members of the CUNY Justice Academy, our partner forensics students who originated at a CUNY community college.

DSC05383During this winter RTW, Professor Rauceo and Professor Roberts addressed PRISM expectations and the important of maturity and integrity when performing research.  Visiting speakers also included PRISM mentors (Champeil, Rosati, Delgado-Cruzata, and Brown) who briefed students on their lab’s projects and opened the door for these new students to inquire about open researcher spots.

DSC05450An important component of this and every RTW is the time that they spend in the lab, learning basic techniques related to solutions chemistry.  Prof. Rauceo and Prof. Roberts, along with lab tech, Karol Alvarez, closely supervise these activities, all the while assessing the students’ abilities, in addition to providing feedback about best practices and problem solving strategies.

PRISM coordinators Dr. Ed Sanabria-Valentin and Fran Jimenez met with students throughout the RTW to speak about PRISM policies, procedures, events, and opportunities for stipends and travel funding.  The new recruits were able to interact quite a bit with our veteran peer mentors, who were kind enough to share their best tips for choosing a mentor, time management, and do’s and don’ts of undergraduate research from a more relatable perspective.

We wish our new students a great deal of success as they get matched with a professor and embark on their undergraduate research careers!  Click here for more pictures from this training event.

12 PRISM students attend ABRCMS ’14 in San Antonio, TX

GroupThe 14th Annual Biomedical Research Conference for Minority Students (ABRCMS) was held in San Antonio, Texas earlier this month (November 12-15). Twelve PRISM students attended, presenting posters about their scientific research at John Jay. Three of these students (Yessenia Lopez, Porfirio Fernandez, and Jiwon Seo) received Travel Awards ABRCMSfrom the conference to defray the costs of their travels. Dr. Jason Rauceo and Dr. Garry Brown from the Science Department accompanied the students, as well as PRISM coordinator Dr. Edgardo Sanabria-Valentin.

ABRCMS is one of the largest undergraduate research conferences in the United States, with more than 1,700 students presenting their research in about twelve fields in biomedical sciences. Our students presented their work in diverse projects (chemistry, toxicology, cellular and molecular biology, environmental sciences, and microbiology), showcasing the diversity of scientific research being performed at John Jay.Stephania

To prepare for their presentation, PRISM organized “ABRCMS Boot Camp” the week before departing for Texas. Students got to practice their presentation skills in daily sessions helped by faculty members of the Science Department and the PRISM staff. They also got to learn about networking and perfected their “elevator pitch.” At the conference they attended sessions discussing the importance of science communication, how to prepare an application to graduate programs, the different types of graduate programs, and the importance of networking and other professional development events.

An account by Richard Khusial, senior Forensic Science major:

Continue reading

The Woodrow Wilson Teaching Fellowships

The Woodrow Wilson Teaching Fellowship seeks to attract talented, committed individuals with backgrounds in the STEM fields—science, technology, engineering, and mathematics—into teaching in high-need secondary schools in Georgia,Indiana, Michigan, Ohio, and New Jersey. Eligible applicants include current undergraduates, recent college graduates, mid-career professionals, and retirees who have majored in, or had careers in, STEM fields.



The Fellowship also works to change the way top teachers are prepared, partnering with colleges and universities that have agreed to provide Fellows with innovative, year-long classroom experiences, rigorous academic work, and ongoing mentoring.

The Teaching Fellowship includes:

  • admission to a master’s degree program at a partner university
  • preparation for teacher certification in science, mathematics or technology education
  • extensive preparation for teaching in a high-need urban or rural secondary school for one full year prior to becoming the teacher-of-record in a science or math classroom
  • a $30,000 stipend, with tuition arrangements varying by campus in Georgia, Indiana, Michigan, Ohio, and New Jersey. (Once Fellows are certified teachers at the end of the first year, they obtain salaried employment in high-need schools.)
  • support and mentoring throughout the three-year teaching commitment
  • support of a cohort of WW Fellows passionate about science and math education
  • lifelong membership in a national network of Woodrow Wilson Fellows who are intellectual leaders

There are two upcoming application deadlines – November 14, 2014 and January 31, 2015 (the final deadline).  For more information and to apply, please go to The Woodrow Wilson Teaching Fellowship website.

NYC Teaching Fellows Program

The New York City Teaching Fellows program is preparing a critical mass of exceptional teachers committed to a better future for the NYC students who need them most.  Fellows complete a master’s degree that is subsidized by the NYC Department of Education while teaching full-time in a public school, allowing for a direct transition into the classroom.

Since 2000, the Fellows program has provided New York City students with thousands of talented new teachers. Today, Fellows work in 80 percent of New York City’s 1,800 public schools and represent 12 percent of the city’s active teaching force.

The priority application deadline is November 6th, 2014.

Here’s How to Get Started:

  1. Visit the NYC TF website to see if you are eligible to apply.
  2. Visit the FAQ page to learn about the program and the Fellowship experience.
  3. Review the application process and learn about the materials you’ll need to gather.

Attend an Event: If you would like to attend an online information session to receive more information about the NYC TF program, please register here. The next online information session will be on Thursday, October 30th from 6pm-7pm EST.

America Needs You Fellowships

America Needs You (ANY) fights for economic mobility for ambitious, low-income college students. Through intensive career development, mentorship, and professional networks, ANY enables students to realize their academic and career aspirations. ANY’s Fellows Program is an intensive program for high-achieving, low-income, first-generation college students.

Outcomes of the ANY Fellows from the 2013 annual report.

Outcomes of the ANY Fellows from the 2013 annual report.

The two-year program offers students (Fellows) career development workshops, access to internships, and professional development grants. All Fellows are matched one-on-one with a successful and motivated professional “Mentor Coach” dedicated to their personal and professional growth. Mentor Coaches and Fellows work together to cultivate the Fellows’ strengths and interests, and direct them into successful careers.


  • Earn up to $2,500 in professional development grants
  • Access 2 summer internships at prestigious companies and organizations
  • Receive individualized support from a Mentor Coach
  • Obtain in-kind services and products such as free business attire and subsidized test preparation
  • Network with leading industry professionals

Fellows attend 28 full-day workshops over two years, receiving intense career development and leadership training. Workshops cover topics such as resumes, cover letters, interviewing, public speaking, and relationship management. All workshops take place on Saturdays and typically run from 10:00am to 4:00pm.

To learn about eligibility requirements and apply for the Fellows Program, go to either New York Needs You or New Jersey Needs You.